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FUJIFILM wako | L-012化學(xué)發(fā)光探針

L-012化學(xué)發(fā)光探針



生化學(xué)用 for Biochemistry3116094040028274.png

規(guī)格含量 : 90.0+%（HPLC）

制造商 : FUJIFILM Wako Pure Chemical Corporation（富士膠片和光）

儲存條件：冷藏（干冰運輸）

CAS RN? : 143556-24-5   

分子式 : C13H8ClN4NaO2  

分子量 : 310.67

3116094040028274.png


◆基本信息


L-012 是一種高靈敏度的化學(xué)發(fā)光（CHL）探針，比魯米諾（Luminol）試劑更有效。L-012 與多種類型的活性氧反應(yīng)，這些活性氧來自人血液和口腔中激活的中性粒細(xì)胞或者大鼠腹膜腔。該產(chǎn)品可以用于有辣根過氧化物酶的ELISA 試劑盒，可提高反應(yīng)靈敏度。


結(jié)構(gòu)式

 

溶解及保存：蒸餾水溶解成20 mM/L（6.23 mg/mL）的水溶液，再用50 mM/L的Tris鹽酸緩沖液（pH 7.5）稀釋 至最終濃度為500 μM/L（有細(xì)胞的反應(yīng)體系）。另外，在沒細(xì)胞參與的反應(yīng)體系中時，建議使用終濃度為5~50 μM/L。母液冷凍保存，使用時再稀釋。



◆物性信息


外觀

白色~黃色，結(jié)晶~粉末

溶解性

可溶于水。



產(chǎn)品編號產(chǎn)品名稱產(chǎn)品規(guī)格產(chǎn)品等級
120-04891L-012
L-012化學(xué)發(fā)光探針		100 mg－


拜爾迪是FUJIFILM wako授權(quán)代理商，

更多產(chǎn)品請咨詢：

北京拜爾迪生物技術(shù)有限公司

咨詢熱線：010-62960866


Q&A


Q： L-012能滲入細(xì)胞嗎?

A： 由于L-012對脂質(zhì)高度親和，它比luminol和lucigenin更能具有細(xì)胞滲透性。另一方面，ROS無限制地在胞外釋放。因此L-012被認(rèn)為也能

       檢測胞外ROS。

 

Q： L-012的使用濃度如何?

A： 這取決于應(yīng)用和條件。

       [體外] 培養(yǎng)細(xì)胞（EoL-1細(xì)胞）： 500 ~ 800 μmol/L.

       全血和分離的中性粒細(xì)胞： 400 μmol/L

       多形核中性粒細(xì)胞（PMNs）（由小鼠中分離）：  2 mmol/L

       無細(xì)胞的樣品： 4 μmol/L, 15 μmol/L, and 50 μmol/L

       [體內(nèi)] Balb/c 小鼠[皮下]： 20 ~ 80 μL of L-012(5mg/mL)

       Balb/c 小鼠[腹膜內(nèi)]： 100 μL of L-012 (15mg/mL)

       C57BL/10.Q (B10.Q) 小鼠 [腹膜內(nèi)]： 1 ~ 75 mg/kg

 

Q： 是否可用于動物活體成像？

A： 如果所用的小動物成像儀中有發(fā)光檢測探頭，就可以進(jìn)行檢測。以下是文獻(xiàn)報道過的儀器型號：

      ?IVIS 100 imaging systems

      ?Infinite M200 [Tecan]
      ?Luminescence Reader BLR-201 [Aloka]
      ?Beckman-Coulter DTX880 multimode plate reader [Beckman]

 

Q：  是否可用于固定細(xì)胞？

A：  目前尚無證據(jù)表明，活性氧（ROS）在細(xì)胞活性停止后仍然存在。普遍看法是認(rèn)為在細(xì)胞活性停止后（細(xì)胞死亡）ROS消失。固定組織或

        細(xì)胞會停止細(xì)胞活性，因此不建議用于ROS檢測。

        本品無需固定檢測對象即可檢測到ROS，使用方法包括但不限于將其添加到細(xì)胞培養(yǎng)上清液中或進(jìn)行動物腹腔注射。



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